Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2015 Apr 29.

Published in final edited form as: Circulation. 2014 Feb 11;129(17):1742–1750. doi: 10.1161/CIRCULATIONAHA.113.008452

Microtubule polymerization accelerates JP2 redistribution in response to stress. A. Representative confocal images of in situ JP2 immunofluorescent staining in LV cryosections from age-matched sham and TAB mice injected with saline or colchicines (Colch). (n=3–4 hearts per group). B. Representative in situ JP2 immunofluorescence in LV cryosections from human “healthy” donor hearts (n=3), and hearts with ischemic (n=4) or dilated cardiomyopathy (n=3). 10–15 confocal images were acquired from each heart sample. C. Representative images of isolated cardiomyocytes stained with anti-JP2 antibody after treatment with 10 μM nocodazole, 20 μM taxol, or the combination of both in culture for 48 or 72 hrs. D. Average JP2_power in cultured cardiomyocytes with different treatments. E. Semi-quantitative analysis of JP2 redistribution from T-tubule membrane to periphery sarcolemma. Con: DMSO control; Noc: nocodazole. n = 15–20 cells per group.* p<0.05 vs control at 0 hr; † p<0.05 vs control at 48 hrs; ‡ p<0.05 vs control at the same time point; ξ p<0.05 vs Taxol of the same time point; φ p<0.05 vs Noc at the same time point. p<0.001 among Con, Noc, Taxol, and Noc+Taxol groups at 48 hrs and 72 hrs (by Linear mixed-effects model).

Microtubule polymerization accelerates JP2 redistribution in response to stress. A. Representative confocal images of in situ JP2 immunofluorescent staining in LV cryosections from age-matched sham and TAB mice injected with saline or colchicines (Colch). (n=3–4 hearts per group). B. Representative in situ JP2 immunofluorescence in LV cryosections from human “healthy” donor hearts (n=3), and hearts with ischemic (n=4) or dilated cardiomyopathy (n=3). 10–15 confocal images were acquired from each heart sample. C. Representative images of isolated cardiomyocytes stained with anti-JP2 antibody after treatment with 10 μM nocodazole, 20 μM taxol, or the combination of both in culture for 48 or 72 hrs. D. Average JP2_power in cultured cardiomyocytes with different treatments. E. Semi-quantitative analysis of JP2 redistribution from T-tubule membrane to periphery sarcolemma. Con: DMSO control; Noc: nocodazole. n = 15–20 cells per group.* p<0.05 vs control at 0 hr; † p<0.05 vs control at 48 hrs; ‡ p<0.05 vs control at the same time point; ξ p<0.05 vs Taxol of the same time point; φ p<0.05 vs Noc at the same time point. p<0.001 among Con, Noc, Taxol, and Noc+Taxol groups at 48 hrs and 72 hrs (by Linear mixed-effects model).