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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Free Radic Biol Med. 2014 Jan 17;70:155–166. doi: 10.1016/j.freeradbiomed.2014.01.015

Figure 3.

Figure 3

Nrf2-knockdown impairs the antioxidant response following CSE, with increased cellular superoxide anion. Cells transfected with 15nM non-targeting siRNA control (CTLR) or Nrf2 siRNA were treated with DMSO vehicle (control) or 125μg/ml CSE for 24h. A) Gene expression of Nrf2 and NQO-1. Changes are presented as fold change over vehicle control. B) Superoxide anion, measured by DHE assay, in ARPE-19 cells after 125μg/ml CSE. C) Graph showing decreased cellular total glutathione by 125μg/ml CSE is magnified by Nrf2-KD. D) Graph showing increased protein carbonylation by125μg/ml CSE is magnified by Nrf2-KD. E) Graph showing decreased NAPDH oxidoreductase activity by 125μg/ml CSE is magnified by Nrf2-KD compared to siRNA control with CSE. Note: B–E, since values for vehicle control and siRNA CTRL were similar, the vehicle control bars were omitted. For all assays, n=3 ind exp. *p<0.05, **p<0.01, ***p<0.001.