Fig. 1.

MHC class I APM component down-regulation in DCs co-incubated with RM1 prostate cancer cell line. DCs from bone marrow precursors (10⁶ cells/well) were generated with GM-CSF and IL-4 as described in “Materials and methods”. RM1 cells (10⁶ cells/well) were added in a transwell system and incubated for the first 3 days in cultures. Tumor-treated and control DCs were intracytoplasmatically stained with delta-, MB-1-, LMP-10-, ERp57-, and tapasin-specific Ab, and analyzed by flow cytometry. Data represent the percentage of positive cells and MFI. The results of one representative out of three independent experiments are shown