Fig. 2.
RM1 cells inhibited antigen-presenting capacity of DCs. DCs from murine bone marrow precursors were generated as described in the Fig. 1 legend. RM1 cells were added in a transwell system and incubated for the first 3 days in cultures. Tumor-treated and control 6-day-old DCs were loaded with OVA overnight, washed and cultured in 96-well flat-bottom plates in complete medium with OVA257–264-specific T cells (B3Z) as described in “Materials and methods”. The ability of DCs to present OVA antigen to specific T cells was determined as IL-2 production by activated T cells and assessed by ELISA. Data represent the mean ± SEM of triplicate measurements from three independent experiments. * P < 0.05