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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Cancer Res. 2014 Mar 31;74(8):2295–2305. doi: 10.1158/0008-5472.CAN-13-1803

Figure 6. Disruption of xC− transporter function resulted in partial suppression in IGF-I-induced cell proliferation and sensitized cells to IGF-IR inhibitors.

Figure 6

(A) MCF-7 cells were first pretreated with SASP (0.1 mM) for 48 h or NAC (0.1 mM) for 24 h in SFM and then treated with IGF-I (5 nM) for 5 days. Cell viability was determined by performing MTT assay. (B) MCF-7 cells were infected by either scrambled shRNA or xCT specific shRNA to generate stable xCT down-regulation clone. Cells were grown in SFM for 24h then treated with IGF-I (5 nM) for 3 days or 5 days. Cell monolayer growth was measured by MTT assay. (C) MCF-7 cells were treated with indicated treatment combinations. Anchorage independent growth was determined after 14 days. Data are mean ± SEM; all results are representative of three independent replicates.