TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| XL-1Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIqZΔM15Tn10(Tetr)] | 43 |
| DH5α | endA1 gyrA96 thi-1 hsdR17 supE44 relA1 recA1 ΔlacU169(φ80ΔlacZΔM15) | 43 |
| A118 | Chromosomally located Tn5lac | 45 |
| H1443 | K-12 strain, aroB | 17 |
| RK4375 | fhuC::Tn10 fepA9 aroE24 (previously fhuB478::Tn10)b | E. coli Genetic Stock Center |
| S. meliloti | ||
| 2011 | Wild typec; Smr Nod+ Fix+ | |
| 2011rhtX1 | 2011 with Ω-Km cassette in rhtX (rhtX1) | This study |
| 2011rhtX43 | 2011 with Tn5lac insertion in rhtX (rhtX43) | This study |
| 2011Sma2335km | 2011 with Kmr cassette in Sma2335 | This study |
| 2011rhtA45 | Tn5lac insertion in rhtA | 30 |
| 2011rhrA26 | Tn5lac insertion in rhrA | 30 |
| 102F34 | Does not produce or utilize rhizobactin 1021; Nod+ Fix+ | 41 |
| P. aeruginosa | ||
| PA01 | Wild type; produces pyoverdine and pyochelin | |
| CDC5 | pvd-2; produces pyochelin, does not produce pyoverdine | 2 |
| DH143 | pvd-2 fptA | 19 |
| PA4218-km1 | CDC5 with a Kmr cassette in fptX (PA4218) | This study |
| B. megaterium ATCC 19213 (91-02) | Schizokinen producer | NCIMBd culture collection |
| Plasmids | ||
| pJQ200ks | Gmrmob sacB | 39 |
| pRK600 | Tra Cmr | 15 |
| pCR2.1 | Apr | Invitrogen |
| pMO 012405 | Cosmid carrying genomic region of PAO1 including fptX coding sequence | Pseudomonas Genetic Stock Center |
| pSUP104 | Tcrmob | 38 |
| pBBR1MCS-5 | Gmrmob | 25 |
| pPOC4 | pBBR1MCS-5::rhtX | This study |
| pPOC5 | pSUP104 with the region carrying rhrA and rhtA | This study |
| pEN7 | iutA | 18 |
| pRG13 | iucABCD | 18 |
| pHP45Ω-Km | Source of Ω-Km | 13 |
| pUC4K | Source of Kmr cassette | Amersham |
| pPC35 | pCR2.1 fhuCDB | This study |
| pPC29 | pCR2.1 fhuCD | This study |
| pPC20 | pCR2.1 fhuDB | This study |
a
Apr, Cmr, Gmr, Kmr, Smr, and Tcr, ampicillin, chloramphenicol, gentamicin, kanamycin, streptomycin, and tetracycline resistance, respectively; Tra, transfer.
b
The strain carries the mutation previously described as fhuB478::Tn10 by Kadner et al. (22), which was determined to be fhuC::Tn10 in this work. See text for details.
c
S. meliloti 2011 is the parent strain of 1021 (31). 1021 was used to sequence the genome of the species and for the purification and chemical characterization of rhizobactin 1021 (35).
d
NCIMB, National Collections of Industrial, Food and Marine Bacteria.