Figure 1.

Analysis of the plasmablast antibody repertoire of three individuals vaccinated against influenza. (A-C) The paired heavy-chain and light-chain antibody sequences expressed by individual plasmablasts were arranged by heavy chain V gene family usage and clustered to generate the displayed phylogenetic trees. Each phylogenetic tree represents an antibody repertoire from a different influenza vaccine recipient. Antibodies from clonal families are shown in bold. The letter F followed by a number denotes an antibody that was selected for cloning and expression; circles denote antibodies that bound components of the influenza vaccine, and squares denote antibodies that did not (see Fig. 3). The circles and squares are colored green if they denote antibodies from clonal families, and blue if they denote singleton antibodies.