Figure 1.
M2-10B4 cells can be used to generate MCMV plaques. A) Six-well plates were seeded with 5 × 105 M2-10B4s one day before infection. Cells were infected with BAC-derived MCMV (MW97.01 [17]) for 1.5 hours, overlaid with high viscosity CMC mixture (1600 mPas final viscosity), and incubated for 6 days. Monolayers were fixed and stained with crystal violet solution as described. Representative images are shown. Sizing bars = 1mm in each image. B) M2-10B4 cells or Balb-3T3s were seeded in replicate wells and live cells were harvested and counted on the indicated days.