Figure 4.

Expression of BMP-2 in 4 groups of MG-63 cells. (A) Densitometry of the RT-qPCR products normalized to GAPDH. BMP-2 expression was increased in both CGRP and CGRP+Noggin groups, with significant differences when compared with the Noggin and control groups (P<0.01). (B) Western blot for BMP expression in the different treatment groups; β-actin was used as an internal control. (C) Western blots were quantified via densitometry scanning using NIH Image software and plotted in histograms representing mean±SD. Data were obtained from 4 independent experiments.