Figure 3.

Differentiation of human neural stem/progenitor cells cultured on substrates coated with short IKVAV peptides and whole LN at the day 14. (A): Cells were stained with DAPI (blue), GFAP (green), and β-III-tubulin (red). Scale bars = 100 μm. (B): Qualification of cells that differentiated into neurons (β-III-tubulin-positive) and glial cells (GFAP-positive). Our short IKVAV peptide induced significantly higher rate of differentiation into neurons and lower rate to glial cells compared with LN (∗,∗p < .05). Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; GFAP, glial fibrillary acidic protein; IKVAV, Ile-Lys-Val-Ala-Val sequence; LN, laminin.