Skip to main content
. 2008 Dec 15;30(1):98–106. doi: 10.1038/aps.2008.11

Figure 8.

Figure 8

Effect of PTX on VEGF mRNA stability. Overnight serum deprived HK-2 cells were treated with PTX of 0.5 g/L for 24 h before treatment with 5 μmol/L actinomycin D. VEGF mRNA levels at 0, 0.5, 1, 1.5, 2, and 4 h after addition of actinomycin D were assessed by RT-PCR. The half-life (T1/2) of VEGF mRNA was calculated from the slope of the plot of versus time of decay, using linear regression analysis. The estimated half-life of VEGF mRNA was 0.715±0.094 h. PTX treatment prolonged the half-life with increase by 1.07 fold. P<0.01 vs control.