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. 2008 Dec 15;30(1):120–124. doi: 10.1038/aps.2008.9

Figure 2.

Figure 2

Effect of NYGGF4 on GLUT4 translocation. 3T3-L1 preadipocytes transfected with NYGGF4 or the empty vector were induced to differentiate. Membrane proteins and total proteins were extracted from the differentiated cells incubated with or without 100 nmol/L insulin for 30 min. Immunoblotting was performed using antibodies against GLUT4. The results are representative of those obtained from three independent experiments.