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Experimental protocol. All experimental groups began with a 20 min perfusion period to allow for stabilization of the isolated hearts. Then, the hearts were divided into the normal control group (A1), the ischemia control group (A2) and the PSS treatment group (A3– A8), which received different concentrations (0.3, 1, 3, 10, 30, or 100 mg/L) of PSS reperfusion for 60 min.
