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BCG treatment upregulates the production of IL-10 and TGF-β by OVA-stimulated splenocytes in vitro. Culture cell-free supernatants were harvested and analyzed for cytokine release by ELISA. Results are expressed in pg/mL. Each condition was cultured in triplicate wells in each experiment. Data represent mean±SEM from 12 mice for each group. bP<0.05 BCG-treated groups vs OVA group or control group. eP<0.05 OVA group vs control group.
