Beclin1 expression and LC3-II/LC3-I in ipsilateral hemisphere. (A) Diagram of the brain section. ROIs were defined in the ipsilateral SVZ (a), Ctx (b), and St (c). (B) Western blot analysis of Beclin1, LC3-II, and LC3-I expression in ipsilateral cortex, striatum and DG at different times after reperfusion, as described in the text. Results were obtained from three independent experiments; only one representative experiment is shown. (C and D) Bar graphs show quantitative evaluation of Beclin1 expression and LC3-II/LC3-I in the ipsilateral hemisphere. n=3. Mean±SD. A nonparametric test was used for statistical analysis. bP<0.05, cP<0.01 compared with sham group. Ctx, Cortex. St, Striatum. DG, dentate gyrus.