Figure 2.

Ultrastructural morphology of several autophagic profiles. (A) An early stage of autophagic vacuole formation, identified as membranes from the rough endoplasmic reticulum containing cytosolic components (white asterisks) in neurons from the DG at 10 h after reperfusion. This process leads to the formation of the autophagosomes, which are characterized by double membrane vesicles (white arrows). (B) An autophagosome with several double membranes in a neuron of the DG at 24 h after reperfusion (white asterisk). (C) Autophagosomes, adjacent to lysosomes (white arrows), contain some vacuoles bounded by a small inner vesicle enclosing residues of the lytic organelles (black asterisks) (36 h after reperfusion). (D) Autophagic vacuole with double membrane vesicles (black asterisk) adjacent to lysosomes (white arrowhead). Autophagolysosomes contain residues of membranes and lytic organelles (white arrows) (48 h after reperfusion). Scale bar: 500 nm.