Figure 3.

Liquiritigenin protects neuron cells against Aβ_25–35-induced apoptosis. (A) Nuclei were stained with fluorescent dye Hoechst 33342 for assessment of apoptosis; arrowheads indicate apoptotic cells. Magnification: ×200. (B) Representative FCM histograms stained by Annexin V-FITC/PI. Annexin V⁺ and PI⁻ cells were designed as apoptotic. (C) Apoptotic rate of cells, determined by FCM. Bars represent mean±SEM (n=4). ^aP>0.05, ^bP<0.05, ^cP<0.01 vs control; ^dP>0.05, ^eP<0.05, ^fP<0.01 vs Aβ_25–35 alone.