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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Free Radic Biol Med. 2009 May 27;47(4):458–467. doi: 10.1016/j.freeradbiomed.2009.05.019

Fig. 2. N-nitrosation in the XO/HX/SPENO system.

Fig. 2

(A) DAN (100 μM) derived fluorescence was monitored at constant SPENO (30 μM) and increasing XO concentrations in 0.1 M potassium phosphate buffer, pH 7.4, containing 1 mM HX at 37°C. Data were collected after 5 min incubation. (B) DAN (20 μM) fluorescence was monitored at constant SPENO (10 μM) and increasing XO concentrations in 0.1 M potassium phosphate buffer, pH 7.4, containing 1 mM hypoxanthine at 37°C. Data were collected after 25 min incubation and are expressed as the means±SEM of two or three independent experiments.