Figure 3.

PSA inhibited MOG_35–55-specific cytokine production and cell proliferation. (A) Time course of IL-17 production during EAE. Draining lymphocytes (DLNs) from immunized mice were isolated at the indicated time points and cultured with or without MOG_35–55 (20 μg/mL). Antigen-specific IL-17 production was measured at 96 h by ELISA. (B) On day 12 pi, DLNs from vehicle and PSA treated group (five mice per group) were incubated with or without MOG_35–55 (20 μg/mL) for 72 h. Cultures were pulsed with 0.5 μCi/well [³H]-Thymidine for the final 12 h. (C, D) Culture supernatants were collected at 48 h to measure IL-2 and IFN-γ level by ELISA. (E) DLNs from immunized mice treated with vehicle or PSA were isolated at the indicated time points and cultured with MOG_35–55 (20 μg/mL). Antigen-specific IL-17 production was measured at 96 h by ELISA. (F) DLNs from immunized mice treated with vehicle or PSA were isolated at the indicated time points and cultured with MOG_35–55 (20 μg/mL). Antigen-specific IL-6 production was measured at 48 h by ELISA. Results were expressed as mean±SEM. ^bP<0.05, ^cP<0.01 compared with vehicle control (unpaired Student's t-test). Three independent experiments were performed with similar results.