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. 2009 Jul 13;30(8):1144–1152. doi: 10.1038/aps.2009.101

Figure 5.

Figure 5

PSA reduced the proportion of IL-17 producing T cells in MOG-immunized mice. (A) T cells were prepared from lymph nodes by eliminating B cells and APCs. An APC-enriched population was prepared from spleen cells by eliminating T and B cells. T cells (4×106/well) were cultured with APCs (1×106/well) in the presence of 20 μg/mL of MOG and 20 ng/mL of rmIL-23 for 96 h to assess the intracellular proportion of IL-17 producing T cells. Numbers in quadrants indicate the percentage of positive cells in each. (B) DLNs from vehicle and PSA treated mice were harvested on d 12 pi and cultured in the presence of MOG35–55 and rmIL-23 for 4 d. Purified T cells were transferred to naïve C57BL/6 mice (1×107 cells/mouse). Mice were scored daily for clinical signs of EAE up to 21 d after cell transfer. Results were expressed as mean±SEM. bP<0.05, cP<0.01 compared with the vehicle control (unpaired Student's t-test). Three independent experiments were performed with similar results.