Table 2.

PCR primers and hybridization probes used in estrogen receptor-α genotyping by real-time PCR combined with melting curve analysis

Polymorphism site	Melting Temperature (°C)	Oligonucleotide sequences
Exon 1/codon 10
TCT	56	Primers for PCR reaction:
TCC	64	Sense: 5'-GGTTTCTGAGCCTTCTGCCCTG-3' (301–322)
		Antisense: 5'-AGGCCGGTCTGACCGTAGA-3' (593–575)
		Hybridization probes:
		Sensor: 5'-ACACCAAAGCATCCGGGATGred-3' (377–396)
		Anchor: 5'-fluoresceinCCCTACTGCATCAGATCCAAGGGAACG-3' (398–424)
Exon 4/codon 325
CCC	58.5	Primers for PCR reaction:
CCG	65.5	Sense: 5'-ACCTGTGTTTTCAGGGATACGA-3' (336–357)
		Antisense: 5'-GCTGCGCTTCGCATTCTTAC-3' (705–686)
		Hybridization probes:
		Sensor: 5'-GCTGAGCCCCCGATACTCTAred-3' (554–573)
		Anchor: 5'-fluoresceinCCGAGTATGATCCTACCAGACCCTTCA-3' (576–602)
Exon 8/codon 594
ACG	61	Primers for PCR reaction:
ACA	67	Sense: 5'-CTGTGTCTTCCCACCTACAG-3' (337–356)
		Antisense: 5'-GGGTAAAATGCAGCAGGGATT-3' (641–621)
		Hybridization probes:
		Sensor: 5'-TCCCTGCCACAGTCTGAGAGCred-3' (575–595)
		Anchor: 5'-fluoresceinCCCTGGCTCCCACACGGTTCAG-3' (597–618)