Figure 2.

Dose- and time-dependent inhibitory effects of silibinin on the invasion and motility of ARCaP_M cells. ARCaP_M cells were treated with different concentrations of silibinin (50, 100, and 200 μmol/L) for 24, 48, and 72 h and then harvested and seeded into the upper chamber, either coated or uncoated with Matrigel. The number of cells that invaded or moved into the lower chamber represented the invasion and motility capabilities. (A) Invasion and motility inhibition of ARCaP_M cells after 100 μmol/L silibinin treatment for 48 h (magnification×100). (B and C) Silibinin inhibited the invasion and motility of ARCaP_M cells in a dose- and time-dependent manner. Data represent means±SEM of three independent experiments.