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Dose- and time-dependent inhibitory effects of silibinin on migration of ARCaPM cells in vitro. The confluent ARCaPM monolayers were wounded by scraping and treated with silibinin (50, 100, and 200 μmol/L). Cell migration to the wound surface was monitored after 24, 48, and 72 h. A representative image is shown (magnification×100), and the average migration distances of the wound edge in three independent experiments are quantitated below the photograph data. Bar represents 200 μm.
