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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: J Biomol Screen. 2013 Nov 26;19(4):556–565. doi: 10.1177/1087057113513640

Figure 5.

Figure 5

Pilot screen of the NIH Diversity Set III using the Nef-BiFC assay. The Diversity Set III of ~ 1600 compounds was screened for inhibitors of Nef dimerization using the Nef-BiFC assay as described under Materials and Methods. The resulting data set was ranked according to (A) BiFC/mRFP ratio, (B) control percent inhibition (C) Z score and (D) median pseudo percent inhibition. The dashed line represents the cut-off threshold for potential hits within each ranking. All four approaches identified the same compounds as prospective hits (red circles). The identities of these compounds are presented in supplemental Table S2.