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. 2011 Sep 11;8(10):853–859. doi: 10.1038/nmeth.1703

Figure 1. Transgenic feline embryo generation.

Figure 1

(a) Optimized transgenesis protocol. PMSG, pregnant mare serum gonadotropin; HCG, human chorionic gonadotropin; IU, international units; IVM, in vitro maturation; IVC, in vitro culture. (b) Transgene expression in hatching feline blastocysts developed in vitro after pre-IVF lentiviral vector microinjection (top left) of feline oocytes. Living GFP-transgenic cat blastocyst (bottom left) developed from oocyte transduced before IVF with TSinG. Confocal images (right) of fixed transgenic (TBDmGpT) and control (product of untransduced oocytes) blastocysts subjected to immunolabeling show HA-tagged rhTRIMCyp signal (HA); GFP fluorescence; DAPI staining for nuclear DNA and merged images. Scale bars, 100 μm (black bars) and 50 μM (white bars).