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. 2011 Sep 11;8(10):853–859. doi: 10.1038/nmeth.1703

Figure 4. Germline transmission and expression in F1 progeny.

Figure 4

Sperm from the two males (20 months) and a control non-transgenic cat was filtered, pelleted, washed and then purified by the swim-up technique. Sperm genomic DNA was subjected to real time quantitative PCR with primers that amplify the GFP sequence. Images show four F1 progeny of a mating of TgCat1 and TgCat3, imaged for GFP expression; dark fur quenches such that in the black cat only claws were visibly green fluorescent (middle, right).