Table 1. Validation the interaction between the mutants and the SjScrib-PDZ domains by Y2H assays.
| SjScrib-PDZ4 | Ligand sequence and point mutation1 | Y2H2 |
| P1-L8M | CMES(A)HFY* | × |
| P1-L10M | SLQET(A)DIE* | × |
| P1-L23M | FHSYRYTET(A)HIK* | × |
| P1-L34M | YPHSEAMET(A)SIE* | × |
| P1-L35M | SFTEET(A)FIE* | × |
| P1-L44M | LFLTVTNMVAEKPAES(A)EIN* | × |
| P1-L1M | SDNHFLHNVTSGCDLIWDET(A)EIDLE* | × |
| P1-L22M | CDEKYMVVARET(A)NIEMGFPEEASLKK* | × |
| P1-L25M | LNIRPKTIKTLEENLGITIQDIGMGKDFMSKTPKAMATKDKIFFKDPTKGFLQET(A)HFPGNNTNRK* | × |
| SjScrib-PDZ2 | ||
| P2-L1M1 | SNNVNKMKMVLTKKVTEEEVSINRGLLTPKFEDVPRKNTVTEASVTYAFQRGFWELSI(T)* | × |
| P2-L1M2 | SNNVNKMKMVLTKKVTEEEVSINRGLLTPKFEDVPRKNTVTEASVTYAFQRGFWELS(A)I* | × |
| P2-L1M3 | SNNVNKMKMVLTKKVTEEEVSINRGLLTPKFEDVPRKNTVTEASVTYAFQRGFWEL(T)SI* | × |
| P2-L1M4 | SNNVNKMKMVLTKKVTEEEVSINRGLLTPKFEDVPRKNTVTEASVTYAFQRGFWE(A)LSI* | × |
| P2-L1M5 | SNNVNKMKMVLTKKVTEEEVSINRGLLTPKFEDVPRKNTVTEASVTYAFQRGFW(T)ELSI* | × |
| P2-L1M6 | SNNVNKMKMVLTKKVTEEEVSINRGLLTPKFEDVPRKNTVTEASVTYAFQRGF(T)WELSI* | √ |
| P2-L2M1 | CLVTDELWTWTFELML* | √ |
| P2-L2M2 | CLVTDELWTWTFELML(T)E* | × |
| P2-L2M3 | CLVTDELWTWTFELM(T)LE* | × |
| P2-L2M4 | CLVTDELWTWTFEL(T)MLE* | × |
| P2-L2M5 | CLVTDELWTWTFE(A)LMLE* | × |
| P2-L2M6 | CLVTDELWTWTF(T)ELMLE* | × |
| SjScrib-PDZ3 | ||
| P3-L2M | SRKKYIKRWAKDMNRHLSKENIHMPTIRKET(A)FLE* | × |
| P3-L59M | FHFYPISRET(A)WFL* | × |
| P3-L3M | SFQSSKCRVIFKETNIKAEVTT(A)VCVYVCLMSEKEPIDSKRPEFPSLIPPGWASIRDPSSSSCR* | × |
| P3-L34M | GNYT(A)SIIFFPGKKENPKVTR* | × |
| P3-L35M | SSAAGTKHNVST(A)IIFFHPPKQM* | × |
| P3-L41M | NFTT(A)VILLHCQHPDQETEHH* | × |
| P3-L50M | SPGFCLRPSRKCPCGSSKTT(A)VLLWLGNP* | × |
| P3-L51M | SSTLNLVKPHFFET(A)WIFW* | × |
| P3-L56M | WLNSPRLSIGPFTMQNITT(A)VIYFRGFTNSNSVKFSCLSLLSSWDYRHAPPQQANSQA* | × |
| P3-L70M | LRQPEHYWAWGLSPYEYLECLPKDRQKKAQTVRTT(A)IILFHPIHKPS* | × |
| P3-L79M | NFKVFSPLDREKVWFST(A)CILFRRSSEYESSAVNEKIPKLNSTRVGIDTGSIELELQMNRRY* | × |
| P3-L104M | FIRET(A)WVQKSKAVSYISFLIST* | × |
| P3-L117M | NSST(A)FIYLFIFA* | × |
| P3-L134M | FPLKHFTVLHTQST(A)CVLFLLSSHKRT* | × |
| SjScrib-PDZ4 | ||
| P4-L4M1 | LRKITRPHAINNHRPEKEKTYFQYKKELVKSGRGWESSGWQLCA(T)DCLSLIYL* | × |
| P4-L4M2 | LRKITRPHAINNHRPEKEKTYFQYKKELVKSGRGWESSGWQL(T)CADCLSLIYL* | × |
| P4-L4M3 | LRKITRPHAINNHRPEKEKTYFQYKKELVKSGRGWESSGW(T)QLCADCLSLIYL* | × |
| P4-L32M1 | ASALIRYGRKILNSCWCLFRSSFLPGSHECRGVDIHKLWEGKVRNARFYLARPSFVTTFPCGASW(T)* | × |
| P4-L32M2 | ASALIRYGRKILNSCWCLFRSSFLPGSHECRGVDIHKLWEGKVRNARFYLARPSFVTTFPCGA(T)SW* | × |
| P4-L32M3 | ASALIRYGRKILNSCWCLFRSSFLPGSHECRGVDIHKLWEGKVRNARFYLARPSFVTTF(T)PCGASW* | × |
| P4-L39M1 | YSPLPLMARRYKSYLPSGF(T)* | × |
| P4-L39M2 | YSPLPLMARRYKSYLPS(R)GF* | × |
| P4-L39M3 | YSPLPLMARRYKSY(T)LPSGF* | × |
| P4-L8M1 | SIIFSLET(R)* | × |
| P4-L8M2 | SIIFSL(T)ET* | × |
| P4-L8M3 | SIIF(T)SLET* | × |
| P4-L42M1 | CFIMCFVIRKRNGVSFVIAT(R)* | × |
| P4-L42M2 | CFIMCFVIRKRNGVSFVI(T)AT* | × |
| P4-L42M3 | CFIMCFVIRKRNGVSF(T)VIAT* | × |
1
: The potential PBMs within the irregular ligands of each PDZ domain of SjScrib are shown in bold and underline. The substituted residues are indicated in brackets.
2
: √: positive in the Y2H system; ×: negative in the Y2H system.