Table 2. Validation of the predicted ligand candidates of SjScrib-PDZ1 and SjScrib-PDZ4 in the Y2H system.
| Domain | Bait | GenBank Accession No. | Code | C-terminus (S. japonicum) | Protein Description | Y2H# |
| SjScrib-PDZ1 | [E][TS][x][LIF]* | AAX25037 | SP1-1 | RDWLSPETEL* | hypothetical protein | × |
| CAX73718 | SP1-2 | SRFVNFETPF* | Lysosomal protective protein precursor | × | ||
| SjScrib-PDZ4 | [F][x][LIFV][x][LIVA]* | CAX72171 | SP4-1 | ASNASFPLLL* | Cathepsin L | × |
| AAW27318 | SP4-2 | TKDDAFILSI* | hypothetical protein | × | ||
| CAX72944 | SP4-3 | NSIDLFSLPA* | putative TatD DNase domain containing 1 | × | ||
| AAX30137 | SP4-4 | DLGPDFKIIL* | hypothetical protein | × | ||
| AAP06275 | SP4-5 | LLMYIFTLVV* | putative phosphate transporter | × | ||
| CAX75377 | SP4-6 | FLTSAFAVII* | Sorcin | × | ||
| AAW25664 | SP4-7 | EDYIRFTVAI* | similar to calpain | × | ||
| AAC32040 | SP4-8 | SIAIKFDVVL* | prepro-cathepsin C | √ | ||
| AAW25594 | SP4-9 | FGNFLFYIYL* | putative zinc finger protein | × |
#: √: positive in the Y2H system; ×: negative in the Y2H system.