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. 2014 May 1;10(5):e1004347. doi: 10.1371/journal.pgen.1004347

Figure 3. Autophagy is crucial for MetR-mediated longevity.

Figure 3

(A) Chronological aging of MET2 deletion strains carrying an additional gene deletion (Δatg5) and MET+ strain in SCD media supplemented with all aa. Cell survival of 500 cells plated at given time points, normalized to cell survival on day one (n = 6). (B) Chronological aging of MET+ strain treated with indicated amounts of rapamycin (Rap). Cell survival of 500 cells plated at given time points, normalized to cell survival on day one (n = 3; p***). Autophagy was measured by means of ALP activity with a fluorescent plate reader (Tecan, Genios Pro) and normalized to untreated controls at indicated time points (also compare to Figure 4E) (C) (n = 4). (D) Chronological aging of MET+ strain deleted for TOR1. Cell survival of 500 cells plated at given time points, normalized to cell survival on day one (n = 6; p***). (E) Chronological aging of the MET+ strain deleted for TOR1 and ATG5 or ATG5 alone. Cell survival of 500 cells plated at given time points, normalized to cell survival on day one (n = 4–6). See also Figure S4.