Table 1. Analysis of variance (ANOVA) table of sporulation efficiencies in allele replacement strains.
Source of Variation | Df | Sum of Squares | Mean Square Error | F value | P value | Fraction of variance explained (%) |
RME1nc | 1 | 7702.0 | 7702.0 | 864.2 | <2e-16 | 34.32 |
RSF1c | 1 | 4517.0 | 4517.0 | 506.8 | <2e-16 | 20.13 |
IME1c | 1 | 7212.0 | 7212.0 | 809.3 | <2e-16 | 32.13 |
IME1nc | 1 | 1459.1 | 1459.1 | 163.7 | <2e-16 | 6.50 |
RME1nc*RSF1c | 1 | 293.7 | 293.7 | 33.0 | 6.22e-07 | 1.31 |
RME1nc*IME1c | 1 | 134.3 | 134.3 | 15.1 | 0.0003 | 0.60 |
RME1nc*IME1nc | 1 | 84.6 | 84.6 | 9.5 | 0.0034 | 0.53 |
RSF1c*IME1c | 1 | 118.6 | 118.6 | 13.3 | 0.0007 | 0.38 |
RSF1c*IME1nc | 1 | 32.9 | 32.9 | 3.7 | 0.6062 | 0.15 |
IME1c*IME1nc | 1 | 124.6 | 124.6 | 14.0 | 0.0005 | 0.56 |
RME1nc*RSF1c*IME1c | 1 | 161.4 | 161.4 | 18.1 | 9.59e-05 | 0.72 |
RME1nc*RSF1c*IME1nc | 1 | 4.4 | 4.4 | 0.5 | 0.4865 | 0.02 |
RME1nc*IME1c*IME1nc | 1 | 136.3 | 136.3 | 15.3 | 0.0003 | 0.61 |
RSF1c*IME1c*IME1nc | 1 | 34.6 | 34.6 | 3.9 | 0.0546 | 0.15 |
RME1nc*RSF1c*IME1c* IME1nc | 1 | 0.1 | 0.1 | 0.01 | 0.9230 | 0.00031 |
Residuals | 48 | 427.8 | 8.9 | 1.91 |
All experiments were performed in the vineyard strain background. The four sporulation QTN are: RME1nc: RME1(indel-308A), RSF1c: RSF1(D181G), IME1c: IME1(L325M), IME1nc: IME1(A-548G). The source of variation in sporulation efficiency is due to the effect of changing the genotype from the oak to the indicated vineyard allele in the vineyard converted strain (all four oak alleles in the vineyard background). P-values≤0.05 are in bold. Fraction of variance explained: Additive Factors = 93.08%; Interaction Factors = 5.02%.