Table 1. The information of real time PCR primers of target genes detected in mammary epithelial cell from dairy cattle by in vitro culture with different arginine levels.
| Items | Sequences of primers (5′ –3′) | Genebank ID | Production | Annealing temperature |
| CSN1S2 (αs2-casein) | F:AGCTCTCCACCAGTGAGGAA | NM_174528.2 | 150 bp | 56°C |
| R:GCAAGGCGAATTTCTGGTAA | ||||
| CSN3 (kappa-casein) | F:CCAGGAGCAAAACCAAGAAC | NM_174294 | 148 bp | 56°C |
| R:TGCAACTGGTTTCTGTTGGT | ||||
| JAK2 (Janus kinase 2) | F:ACAGGGGCTGGCGTTCA | XM_002689603.1 | 146 bp | 61°C |
| R:TATTGGTAACCAACAGCTCAAGG | ||||
| mTOR (Mechanistic target of rapamycin) | F:ATGCTGTCCCTGGTCCTTATG | XM_001788228.1 | 199 bp | 63°C |
| R:GGGTCAGAGAGTGGCCTTCAA | ||||
| STAT5 (Signal transducer and activatorof transcription 5) | F:AAGACCCAGACCAAGTTCGC | NM_001012673.1 | 203 bp | 64°C |
| R:AGCACCGTGGCAGTAGCAT | ||||
| S6K (Ribosomal protein S6 kinase) | F:GGACATGGCAGGGGTGTTT | NM_205816.1 | 162 bp | 58°C |
| R:GGTATTTGCTCCTGTTACTTTTCG | ||||
| 4EBP1 (4E binding protein 1) | F:GGCAGGCGGTGAAGAGTC | BC120290.1 | 177 bp | 60°C |
| R:CCTGGGCTGCGGGAT | ||||
| GAPDH (glyceraldehyde-3-phosphate dehydrogenase) | F:GGGTCATCATCTCTGCACCT | XM_001252479 | 177 bp | 59°C |
| R:GGTCATAAGTCCCTCCACGA |