Table 3.
mRNA levels of MKI67, ESR1/ERα and the 20 target genes expressed in breast tissues
| Normal breast tissues, group I (n = 8) | ERα-negative breast tumors, group II (n = 5) | ERα-positive breast tumors, group III (n = 17) | Group III vs group I | Group III vs group II | |||||
| Gene | Normal liver tissue | Mean ± SD | Range | Mean ± SD | Range | Mean ± SD | Range | Pa | Pa |
| CYP1B1 | 0.02 | 1.0b ± 0.3 | 0.5–1.3 | 11.2b ± 18.5 | 0.6–43.9 | 2.0b ± 2.8 | 0.3–11.8 | NS | 0.033 |
| CYP2A6 | 9.8 | 1.0 ± 1.2 | 0.2–3.8 | 0.3 ± 0.1 | 0.1–0.4 | 737.9 ± 1931.2 | 0.01–7228.8 | NS | 0.048 |
| CYP2B6 | 0.12 | 1.0 ± 0.8 | 0.1–2.3 | 0.2 ± 0.1 | 0.03–0.24 | 73.3 ± 87.7 | 0.8–249.8 | 0.0023 | 0.0090 |
| CYP2D6 | 5.8 | 1.0 ± 0.6 | 0.3–1.7 | 3.4 ± 2.8 | 1.0–8.3 | 1.0 ± 0.6 | 0.3–2.3 | NS | 0.0054 |
| CYP2E | 0.97 | 1.0 ± 0.4 | 0.6–1.4 | 0.8 ± 0.5 | 0.2–1.4 | 1.5 ± 1.2 | 0.3–4.2 | NS | NS |
| FMO1 | 4.7 | 1.0 ± 0.4 | 0.6–1.4 | 0.6 ± 0.5 | 0.2–1.3 | 0.6 ± 0.5 | 0.1–2.0 | NS | NS |
| FMO5 | 28.5 | 1.0 ± 0.3 | 0.5–1.6 | 1.4 ± 2.1 | 0.2–5.1 | 3.4 ± 3.2 | 0.2–12.2 | NS | 0.042 |
| NAT1 | 1.2 | 1.0 ± 0.3 | 0.5–1.5 | 0.5 ± 0.4 | 0.1–1.0 | 24.1 ± 29.0 | 1.2–105.2 | 0.0031 | 0.0009 |
| COMT | 0.47 | 1.0 ± 0.2 | 0.8–1.3 | 2.2 ± 1.5 | 0.3–4.3 | 0.9 ± 0.5 | 0.3–2.3 | NS | NS |
| NQO1 | 0.009 | 1.0 ± 0.9 | 0.2–2.8 | 0.9 ± 0.9 | 0.1–2.0 | 1.4 ± 1.5 | 0.1–5.2 | NS | NS |
| EPHX1 | 0.75 | 1.0 ± 0.9 | 0.2–2.1 | 0.3 ± 0.3 | 0.1–0.8 | 0.4 ± 0.3 | 0.1–1.0 | NS | NS |
| SULT1A1 | 1.8 | 1.0 ± 0.7 | 0.3–1.6 | 0.6 ± 0.5 | 0.1–1.2 | 1.1 ± 0.9 | 0.4–3.3 | NS | NS |
| SULT2B1 | 0.001 | 1.0 ± 1.0 | 0.0–2.9 | 1.7 ± 1.4 | 0.1–3.3 | 6.9 ± 6.9 | 0.4–24.0 | 0.0094 | NS |
| GSTP1 | 0.54 | 1.0 ± 0.5 | 0.7–1.7 | 2.4 ± 4.0 | 0.2–9.4 | 0.9 ± 0.4 | 0.1–1.6 | NS | NS |
| GSTM1 | 0.27 | 1.0 ± 1.2 | 0.0–2.6 | 0.05 ± 0.11 | 0.0–0.2 | 0.5 ± 1.1 | 0.0–4.3 | NS | NS |
| GSTM3 | 0.23 | 1.0 ± 0.4 | 0.5–1.6 | 0.8 ± 1.3 | 0.1–3.0 | 2.7 ± 2.6 | 0.2–9.3 | NS | 0.011 |
| GSTT1 | 0.35 | 1.0 ± 0.9 | 0.0–2.3 | 0.4 ± 0.3 | 0.1–0.7 | 0.6 ± 0.6 | 0.0–2.2 | NS | NS |
| ABCB1 | 1.6 | 1.0 ± 1.2 | 0.4–2.8 | 0.3 ± 0.1 | 0.01–0.4 | 0.4 ± 0.4 | 0.1–1.3 | NS | NS |
| ABCC1 | 0.16 | 1.0 ± 0.3 | 0.8–1.3 | 1.0 ± 0.7 | 0.3–2.2 | 0.9 ± 0.4 | 0.2–1.7 | NS | NS |
| ABCC11 | 0.25 | 1.0 ± 0.9 | 0.4–3.3 | 31.2 ± 39.0 | 0.1–81.0 | 26.8 ± 36.8 | 0.1–108.3 | 0.039 | NS |
| MKI67 | 70.3 | 1.0 ± 0.9 | 0.2–2.1 | 17.4 ± 6.6 | 10.0–26.6 | 13.0 ± 9.1 | 2.6–31.8 | 0.0023 | NS |
| ESR1/ERα | 0.002 | 1.0 ± 0.3 | 0.6–1.3 | 0.03 ± 0.02 | 0.01–0.05 | 29.6 ± 23.2 | 7.2–76.7 | 0.0023 | 0.0009 |
ERα, estrogen receptor alpha; SD, standard deviation. a P value, Mann–Whitney test. NS, not significant. b Mean of mRNA levels as determined in Materials and methods.