Figure 3. Stability of influenza-specific human CD4 T cells secreting different cytokine combinations.

Human PBMC were stimulated with influenza TIV (A) or SEB (B), and expression of IL-2, IFNγ, IL-4 and IL17a was determined by ICS and flow cytometry. (C) Influenza specific human 2+γ+, 2+γ-, 2-γ+ CD4 T cells were sorted and cultured in neutral conditions for 3 days. The expression of IL-2 and IFNγ was measured by RT-PCR (n = 4). (Representative experiment out of 3 independent experiments).