Skip to main content
. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Dev Biol. 2012 Nov 15;373(2):310–321. doi: 10.1016/j.ydbio.2012.11.004

Fig. 4.

Fig. 4

lola is required cell autonomously in the germline to restrict transit amplification divisions: (A) Percentage of homozygous mutant germ cell cysts in the spermatocyte region of the testis with 16, 32, or greater than 32 germ cells per cyst. Black: FRT42D wildtype control clones. Red: lola5D2 clones. Blue: lolaORE76 clones. n=100 cysts in the spermatocyte region per genotype at 4–6 dpci. (B)–(D): FRT42D control (B), lola5D2 (C), and lolaORE76 (D) homozygous germline clones (yellow outlines). Gray: phase contrast. Green: Ubi-nls-GFP clonal marker. (B) FRT42D spermatocyte cyst containing 16 germ cell nuclei (15 visible in focal plane). (C) lola5D2 spermatocyte cyst containing 32 germ cell nuclei (31 visible in focal plane), with size and morphology characteristic of spermatocytes. (D) lolaORE76 spermatocyte cyst containing more than 32 small germ cell nuclei. Scale bars: 20 μm.