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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Dev Biol. 2012 Nov 15;373(2):310–321. doi: 10.1016/j.ydbio.2012.11.004

Fig. 5.

Fig. 5

lola mutant spermatogonia execute the switch to spermatocyte fate: (A) lolaORE76 mosaic testis stained with anti-Bam. Red: Bam. Green: Ubi-nls-GFP. Blue: DAPI. Yellow outlines: homozygous mutant germline clones. Arrowhead: lolaORE76 spermatogonia with Bam expressed. Arrow: lolaORE76 cyst in the spermatocyte region lacking Bam. Scale bar: 40 μm. (B)–(E): Live squash preparations stained with Hoechst, showing spermatocytes from (B) y-w-, (C) lolaORE76 mosaic, (D) aly, (E) sa testes. Phase contrast (left) and corresponding fluorescent images (right). Yellow outline in (C) homozygous mutant lolaORE76 cyst. (F) lolaORE76 mosaic testis stained with anti-Sa. Red: Sa. Green: pan Lola. Blue: DAPI. Outline: lolaORE76 mutant germ cell cyst. B-F: Scale bars: 20 μm.