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. 2014 May 1;9(5):e96483. doi: 10.1371/journal.pone.0096483

Figure 1. Gene editing model system and ssODNs.

Figure 1

The wild-type and mutated eGFP gene segments with the target codon located in the center of the sequences are displayed in green and red respectively. The nucleotide targeted for exchange is emphasized in bold and underlined. Phosphorothioate modified, end protected (denoted with *) 40NT, 72NT, 100NT a 40-mer 72-mer and 100-mer which are used to target the non-transcribed (NT) strand are shown below. Also depicted is the sequence of the 72T, which directs exchange on the transcribed strand (T) of the mutated eGFP gene.