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. 2014 May 1;9(5):e96483. doi: 10.1371/journal.pone.0096483

Figure 3. Fine Mapping of TALEN Cleavage site and Gene Editing Activity.

Figure 3

Unsynchronized HCT116–19 cells were harvested and electroporated at a concentration of 5e5 cells/100 ul with TALENs and the corresponding ODN (40NT, 72NT or 100NT ODN) under the standard reaction ratio TALEN:ssODN (2 µg/1.35 µg). TALEN amounts reflect the total TALEN plasmid added to each sample in equal portions. Following electroporation, cells were placed in 6-well plates and allowed to recover for 48 hours. Analyses took place on a Guava EasyCyte 5HT flow cytometer (see Materials and Methods). Correction efficiency (%) was determined by the number of viable eGFP positive cells divided by the total viable cells in the population. Each treatment was performed in duplicate and error bars represent standard error.