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. 2014 May 1;9(5):e96483. doi: 10.1371/journal.pone.0096483

Figure 5. Analyses of inherent TALEN activity within the eGFP gene region.

Figure 5

(A) Segment of 181 bp PCR product with specific TALEN cut sites indicated by the arrows below and restriction enzyme recognition sequence above. (B) 2% TBE agarose gel analysis of RFLP sites created through site-specific TALEN activity. Expected bands are indicated by arrows: 181 bp for active TALENs, 68+113 for BaeGI (−35), 69+112 for BstNI (−28) and 112+69 for TspRI (+7/8). (C) 2% TBE RFLP analysis of active gene editing site (−1/+1) and controls. Expected bands are indicated by arrows: 181 bp for active TALENs and 95+86 for AvrII (−1/+1). (−1/+1) treated samples were treated with BaeGI and BstNI enzymes to show specificity of TALEN activity, expected bands are indicated by the lower two arrows (68+113, 70+111).