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. 2014 May 1;9(5):e96060. doi: 10.1371/journal.pone.0096060

Figure 3. PTK6 overexpression increases cell migration and invasion of pancreatic cancer cells.

Figure 3

A, Overexpression of PTK6. Cells were transfected with pcDNA3.1-Mock (empty vector) or pcDNA3.1-PTK6. Overexpression of PTK6 in stable trasfectant was confirmed by Western blotting in Panc1 and MIAPaCa2 cells, respectively. The numbers at the bottom indicate the relative intensity of the band for PTK6 to the corresponding controls (normalized by β-actin). B , C , The effect of PTK6 overexpression on cellular migration (B) and invasion (C). Established transfectant cells were evaluated with migration and invasion assays. Forced overexpression of PTK6 increased cellular migration in Panc1 and MiaPaCa2 pancreatic cancer cells (1.6-fold increase for Panc1, and 1.4-fold for MIAPaCa2, respectively, *p<0.05 by t-test). Similarly, forced overexpression of PTK6 increased cellular invasion in these 2 cell lines (1.7-fold increase for Panc1, and 1.6-fold for MiaPaCa2, respectively, *p<0.05 by t-test). Each assay was performed in triplicate.