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. 2014 May 1;10(5):e1004089. doi: 10.1371/journal.ppat.1004089

Figure 3. WTA-mediated adhesion of S. aureus to HNECs and CRNECs.

Figure 3

S. aureus adherence to confluent human nasal epithelial cells (HNECs) monitored under static and mild shear stress condition (0.5 dynes) in 24 well plates or flow chamber assays, respectively. Attachment of the S. aureus wild-type (wt), tagO, and a complemented tagO mutant (tagO compl.) evaluated microscopically by counting at least 6 visible fields per well or chamber slide containing HNECs (A) or CRNECs (B). The data is expressed as means and SEM of 5 independent experiments (static) or of 6 independent experiments (flow). Statistical analysis was performed by one-way ANOVA with Bonferroni's multiple comparison test. Significant differences between groups are indicated by one (P<0.05), two (P<0.01), or three (P<0.001) asterisks (*).