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. 2014 May 1;10(5):e1004089. doi: 10.1371/journal.ppat.1004089

Figure 5. Impact of SREC-I on WTA-mediated adhesion to HNECs and CRNECs.

Figure 5

The anti-human SREC-I antibody was used to test the influence of SREC-I on adhesion of WTA-coated latex beads under static condition in a 24 well microtiter plate assays on HNECs (A) and CRNECs (B). A ratio of 20 beads per epithelial cells was used in all depicted assays. 10 µg/ml of the anti-SREC-I antibody and isotype control were used to test the impact of SREC-I. Adhesion of the beads coated with wt WTA (HNECs and CRNECs) and dltA WTA (HNECs only) or control beads containing no WTA (HNECs and CRNECs) was measured in a fluoreader and expressed as relative fluorescence. The data are expressed as the means and SEM of 6 independent experiments. Statistical analysis was performed by one-way ANOVA with Bonferroni's multiple comparison test (A and B). The dose dependency of WTA-coated latex beads was tested in assays with CRNECs (C). Adhesion of the beads coated with wt WTA and dltA WTA or control beads containing no WTA was measured in a fluoreader and expressed as relative fluorescence. The data are expressed as the means and SEM of 5 independent experiments (C). Significant differences between groups are indicated by one (P<0.05), two (P<0.01), or three (P<0.001) asterisks (*).