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. 2014 May 1;10(5):e1004097. doi: 10.1371/journal.ppat.1004097

Figure 7. CD40L regulates ICOSL expression in macrophages for Tfh-cell development.

Figure 7

(A) Freshly isolated PM of normal and infected mice were stained with F4/80-FITC and CD40-PE and analyzed by flow cytometry. Data are representative of three experiments with 3 mice in each group; (B) PM from normal or infected mice were cultured in vitro for 72 h either in the presence of an agonistic anti-CD40 antibody or its isotype control antibody. The intensity of ICOSL surface expression was measured by flow cytometry after F4/80-FITC and ICOSL-PE staining; (C) Expression of CXCR5 versus PD-1 on CD4+ T cells (gated as CD3+CD4+) after co-culture of normal mice-derived CD4+ T cells with normal or S. japonicum-infected mice-derived macrophages for 3 days with or without SEA in the presence or absence of agonistic anti-CD40 antibody and anti-ICOSL antibody or isotype-matched control antibody. Numbers represent the frequency of the boxed population within the CD4+ T cell population. Data are representative of three experiments with 3 mice in each group.