Skip to main content
. 2014 May 1;10(5):e1004088. doi: 10.1371/journal.ppat.1004088

Figure 7. CovR-D53A and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR.

Figure 7

Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of (A) hasA and (B) prtS. Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% TBE polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.