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Extracts (300 µg of protein) of vector, wtORF1, V125A, and T123D cells were immunoprecipitated with either p110α antibody or Dlg1 antibody as indicated. Recovered proteins, as well as cell extract (input), were analyzed in an immunoblot assay with antibodies to the indicated proteins. Note that V125A and T123D cells express Ad9 E4-ORF1 PBM mutants unable to bind PDZ proteins.
