Figure 11. Sites of E4-ORF1-induced activation of PI3K effector Akt co-localize with Dlg1 at the plasma membrane.

Indirect immunofluorescence assays were performed and analyzed as described in the legend to Figure 8, except the indicated MCF10A lines were dually stained with antibodies to P-Akt (Ser473) (green) and Dlg1 (red). White scale bar = 20 µm.