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. Author manuscript; available in PMC: 2015 Apr 2.
Published in final edited form as: Neuron. 2014 Apr 2;82(1):79–93. doi: 10.1016/j.neuron.2014.02.019

Figure 1. Loss of Mushroom Spines in PS1-M146V KI Hippocampal Neurons.

Figure 1

(A) The spine shape of primary hippocampal neurons from WT or KI mice was visualized with TD-Tomato. Subcellular localization of PSD95 was analyzed by immunostaining of hippocampal cultures. (B) Total spine density and percentage of various spine types in hippocampal neuronal cultures from WT and KI mice. For spine quantification, n=21-23 neurons (from 4 batches of cultures) were analyzed for each group. All the data was collected. (C, E, G). Spine morphology in CA1 hippocampal neurons from 3 months old (C), 6 months old (E) and 12 months old (G) WT and KI mice was visualized by Lucifer yellow injections and 2-photon imaging. (D, F, H) Total spine density and percentage of mushroom (M), stubby (S) and thin (T) spine types in hippocampal neurons from 3 months old (D), 6 months old (F) and 12 months old (H) WT and KI mice. On panels (A), (C), (E) and (G) mushroom spines are marked by arrows; thin spines are marked by triangles; stubby spines are marked by chevron. Scale bars represent 10 μm at panel (A) and 5 μm at panels (C), (E) and (G). n=3 mice for each group. Values are shown as mean ± SEM. * p<0.05, ** p<0.01, **** p<0.0001 by t-test.