Figure 2. Reduced Synaptic Store-Operated Ca²⁺ Entry in PS1-M146V KI Hippocampal Neurons.

(A) Fura-2 fluorescence in live hippocampal neurons. Somatic ROI is shown by a red circle. (B, C) Time course of Fura-2 Ca²⁺ signals (F340/F380) is shown for WT and KI hippocampal neurons following 2 min (B) or 30 min (C) store-depletion protocol. (D) GCaMP5.3 fluorescence in live hippocampal neurons. The samples for synaptic ROI are shown by a red circle. (E) Time course of synaptic GCaMP5.3 Ca²⁺ signals (ΔF/F₀) is shown for WT and KI hippocampal neurons following 30 min store-depletion protocol. On panels (B, C and E) individual cell traces (gray) and average traces (black) are shown for each experimental group. (F) The average peak SOC responses in soma and spines of WT and KI hippocampal neurons (normalized to WT). Values are shown as mean ± SEM (All the data was collected from 3-5 batches of cultures). **p<0.01, **** p<0.0001 by t-test.