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. Author manuscript; available in PMC: 2015 Apr 2.
Published in final edited form as: Neuron. 2014 Apr 2;82(1):79–93. doi: 10.1016/j.neuron.2014.02.019

Figure 3. Downregulation of STIM2 Protein in PS1-M146V KI Hippocampal Neurons.

Figure 3

(A). The expression levels of STIM1, STIM2 and TRPC1 protein were analyzed by Western blotting of lysates from WT and KI hippocampal cultures. Tubulin was used as loading control. ns – non-specific band. (B) Quantification of STIM1, STIM2 and TRPC1 expression levels in WT and KI cultures (normalized to tubulin levels). (C) Subcellular localization of STIM1 and STIM2 were analyzed by immunostaining of hippocampal cultures. MAP2 was used for neuronal labeling. Scale bar: 10μm. (D and E) STIM2 localization in WT and KI neurons transfected with TD-Tomato. Mushroom spines are marked by arrows; thin spines are marked by triangles; stubby spines are marked by chevron. Scale bars in (D): 20 μm in the left panels and 10μm in the right panel. (F) The fraction of STIM2-positive spines is shown for mushroom (M), stubby (S) and thin (T) spines in WT and KI neurons. All the data was collected from 3 batches of cultures. Values are shown as mean ± SEM. * p<0.05, **** p<0.0001 by t-test.