Skip to main content
. Author manuscript; available in PMC: 2014 Aug 3.
Published in final edited form as: Curr Biol. 2014 Jan 23;24(3):347–350. doi: 10.1016/j.cub.2013.12.050

Figure 2. Ascorbic Acid Rescues the Nanog−/− Reprogramming Defect.

Figure 2

(A) Nanog−/− MEFs were reprogrammed in serum/LIF with or without 2i and/or AA as indicated. Resulting dox-independent iPSC colonies were stained for alkaline phosphatase and counted. Results are shown as the average % reprogramming efficiency (iPSC colonies / starting number of MEFs), based on 4 separate replicates +/- 1 S.D. (left panel). Alkaline phosphatase staining of a representative plate is shown (right panel). (B) MEFs (d0 Thy1+) or reprogramming intermediates (Thy1− SSEA-1+) where analyzed by flow cytometry for average % GFP positivity +/- 1 S.D., based on 3 to 5 replicates per time-point. (C) Reprogramming intermediates at the indicated times post dox induction were analyzed by flow cytometry for Thy1, SSEA-1, and EpCAM (left panels) or PECAM1 expression (right panels). Plots are gated on Thy−1SSEA-1+ cells (gray shaded histogram, isotype-matched control antibody). Statistical significance was determined by the Student's T test (* p<0.05; ** p<0.005).