Fig. 2. LCA and LCE inhibit T090-induced SREBP-1c protein and mRNA levels. (A) The effects of LCA or LCE on T0901317 (T090)-mediated SREBP-1c expression in HepG2 cells. Immunoblot analyses were performed on lysates of cells treated with LCA (upper) or LCE (lower) for 1 hr with subsequent treatment with 10 μM T090 for 12 hr. (B) The effects of LCA or LCE on GW3965-induced SREBP-1c expression in HepG2 cells. (C) Real-time RT-PCR assays. HepG2 cells were treated with T090 or vehicle in combination with LCE for 12 hr. The transcripts of SREBP-1c genes were analyzed by real-time RT-PCR assays, with GAPDH used as a reference gene for normalization. (D) Primary mouse hepatocytes were treated with LCE in combination with T090 for 12 hr. The levels of SREBP-1c mRNA were analyzed by real-time RT-PCR assays. Data represent the mean ± S.E.M. of 4 separate experiments. The statistical significance of differences between each treatment group and the control (##p < 0.01) or T090 alone (*p<0.05, **p< 0.01) was determined.